Selective antibody blockade of lymphocyte migration to mucosal sites and mast cell adhesion. Academic Article uri icon

Overview

abstract

  • The integrins alpha4beta7 and alpha4beta1 mediate adhesion to the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) and the vascular cell adhesion molecule-1 (VCAM-1) and are important in T cell and allergic inflammatory reactions in the rat. The relative contributions of alpha4beta7 and alpha4beta1 in these reactions is unknown. To examine the role of alpha4beta7 in the rat a new mAb, TA-6, was developed. TA-6 inhibited adhesion to MAdCAM-1 but not to VCAM-1, a characteristic of alpha4beta7 adhesion, and immunofluorescence and immunoprecipitation studies were compatible with binding to alpha4beta7. TA-6 blocked rat lymphocyte adhesion to mesenteric lymph nodes and T cell migration to mucosal lymphoid tissues and it bound to rat mucosal mast cells. TA-6 did not inhibit lymphocyte adhesion to peripheral lymph nodes and T cell migration to peripheral lymphoid tissues or cutaneous inflammatory sites, and was not expressed on connective tissue mast cells.

publication date

  • May 1, 1999

Research

keywords

  • Antibodies, Blocking
  • Antibodies, Monoclonal
  • Cell Movement
  • Integrins
  • Intestinal Mucosa
  • Lymphocytes
  • Mast Cells

Identity

Scopus Document Identifier

  • 0032934398

Digital Object Identifier (DOI)

  • 10.1002/jlb.65.5.649

PubMed ID

  • 10331494

Additional Document Info

volume

  • 65

issue

  • 5