Cytokine production by CD4+ T-cells responding to antigen presentation by melanoma cells. Academic Article uri icon

Overview

abstract

  • Melanoma cells are unusual because, unlike most epithelial tumours, constitutive expression of HLA class II antigens is common. We have previously demonstrated that a peptide-specific CD4+ T-cell clone proliferates briskly in response to peptide and HLA class II expressing melanoma cell lines derived from metastases. Here we demonstrate that these CD4+ T-cells secrete large amounts of interferon-gamma (IFNgamma) and interleukin-10 (IL10), and insignificant quantities of IL2 or IL4, in response to peptide presentation by both melanoma and autologous B-cells. T-cells produced more IL10 when responding to peptide presentation by melanoma cells compared with B-cells, and less IFNgamma (P<0.01). Addition of IL12 did not alter the cytokines produced but increased the T-cell production of both, especially the production of IL10 in response to peptide presentation by melanoma cells. Our data suggest that differential cytokine production by CD4+ T-cells in response to peptide presentation by HLA class II expressing tumour cells may contribute to tolerance to tumour antigens.

publication date

  • April 1, 1999

Research

keywords

  • Antigen-Presenting Cells
  • CD4-Positive T-Lymphocytes
  • Cytokines
  • Melanoma

Identity

Scopus Document Identifier

  • 0032799369

Digital Object Identifier (DOI)

  • 10.1097/00008390-199904000-00010

PubMed ID

  • 10380940

Additional Document Info

volume

  • 9

issue

  • 2