Binding of extracellular matrix laminin to Escherichia coli expressing the Salmonella outer membrane proteins Rck and PagC. Academic Article uri icon

Overview

abstract

  • Salmonella Rck and PagC are closely related virulence-associated proteins. When expressed in non-adherent, non-invasive laboratory Escherichia coli, both proteins localised to the outer membrane. Only Rck conferred adhesion to culture cells, but both proteins induced bacterial binding to the cell monolayer background, to extracellular matrix (ECM) preparations, and to the ECM component laminin. Laminin binding was saturable and competitive, and was reduced by removal of carbohydrate side chains. Pre-incubation with laminin targeted recombinant Rck and PagC bacteria directly to the eukaryotic cell surface, and eliminated background binding.

publication date

  • July 15, 1999

Research

keywords

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Escherichia coli
  • Laminin
  • Membrane Proteins
  • Salmonella

Identity

Scopus Document Identifier

  • 0033032886

Digital Object Identifier (DOI)

  • 10.1111/j.1574-6968.1999.tb13703.x

PubMed ID

  • 10427733

Additional Document Info

volume

  • 176

issue

  • 2