Up-regulation of MDC15 (metargidin) messenger RNA in human osteoarthritic cartilage. Academic Article uri icon

Overview

abstract

  • OBJECTIVE: The aim of the study was to investigate the messenger RNA (mRNA) expression of the disintegrin metalloproteinase MDC15 (metargidin, or ADAM-15) in normal and osteoarthritic (OA) articular cartilage. METHODS: In situ hybridization experiments and reverse transcription-polymerase chain reaction (RT-PCR) were performed on tissue samples of adult normal and OA articular cartilage. RESULTS: MDC15 mRNA could be detected in normal articular cartilage by RT-PCR using tissue-extracted total RNA as a template. However, the mRNA level remained below the sensitivity of in situ hybridization. In contrast, in situ hybridizations of OA cartilage revealed an intense staining with the MDC15-specific riboprobes. The extension of the analysis to chondrosarcomas showed a strong up-regulation of MDC15 mRNA in these malignant transformed cells. CONCLUSION: Our results demonstrate a markedly strong up-regulation of MDC15 in adult OA and neoplastic cartilage compared with adult normal articular cartilage, indicating a potential role of the disintegrin metalloproteinase in cartilage remodeling.

publication date

  • September 1, 1999

Research

keywords

  • Cartilage, Articular
  • Membrane Proteins
  • Metalloendopeptidases
  • Osteoarthritis

Identity

Scopus Document Identifier

  • 0343618582

Digital Object Identifier (DOI)

  • 10.1002/1529-0131(199909)42:9<1946::AID-ANR21>3.0.CO;2-E

PubMed ID

  • 10513811

Additional Document Info

volume

  • 42

issue

  • 9