A cell line-based neutralization assay for primary human immunodeficiency virus type 1 isolates that use either the CCR5 or the CXCR4 coreceptor. Academic Article uri icon

Overview

abstract

  • We describe here a cell line-based assay for the evaluation of human immunodeficiency virus type 1 (HIV-1) neutralization. The assay is based on CEM.NKR cells, transfected to express the HIV-1 coreceptor CCR5 to supplement the endogenous expression of CD4 and the CXCR4 coreceptor. The resulting CEM.NKR-CCR5 cells efficiently replicate primary HIV-1 isolates of both R5 and X4 phenotypes. A comparison of the CEM.NKR-CCR5 cells with mitogen-activated peripheral blood mononuclear cells (PBMC) in neutralization assays with sera from HIV-1-infected individuals or specific anti-HIV-1 monoclonal antibodies shows that the sensitivity of HIV-1 neutralization is similar in the two cell types. The CEM.NKR-CCR5 cell assay, however, is more convenient to perform and eliminates the donor-to-donor variation in HIV-1 replication efficiency, which is one of the principal drawbacks of the PBMC-based neutralization assay. We suggest that this new assay is suitable for the general measurement of HIV-1 neutralization by antibodies.

publication date

  • November 1, 1999

Research

keywords

  • HIV Antibodies
  • HIV Infections
  • HIV-1
  • Neutralization Tests
  • Receptors, CCR5
  • Receptors, CXCR4

Identity

PubMed Central ID

  • PMC112928

Scopus Document Identifier

  • 0032828730

Digital Object Identifier (DOI)

  • 10.1128/JVI.73.11.8966-8974.1999

PubMed ID

  • 10516002

Additional Document Info

volume

  • 73

issue

  • 11