Parameters influencing measurement of the Gag antigen-specific T-proliferative response to HIV type 1 infection. Academic Article uri icon

Overview

abstract

  • We have analyzed factors that might influence the in vitro quantitation of the T-proliferative response to HIV-1 Gag antigens, a common and increasingly used clinical measurement of helper T cell function in the context of HIV-1 infection. We have compared the rate and extent of T cell proliferation in freshly prepared and previously frozen PBMC samples, and have concluded that frozen cells can be used successfully; we have assessed whether the suppression of any HIV-1 replication in the PBMC cultures affects the extent of T cell proliferation; we have studied which forms of the Gag antigens are the most efficient at inducing T cell proliferation. From the latter experiments, we conclude that Gag proteins that include p17, and perhaps also p7, sequences flanking the central p24 capsid protein, are better stimulants than proteins that comprise only p24 sequences.

publication date

  • February 10, 2000

Research

keywords

  • Capsid Proteins
  • Gene Products, gag
  • HIV Antigens
  • HIV-1
  • T-Lymphocytes
  • Viral Proteins

Identity

Scopus Document Identifier

  • 0034628609

Digital Object Identifier (DOI)

  • 10.1089/088922200309359

PubMed ID

  • 10710214

Additional Document Info

volume

  • 16

issue

  • 3