Physical linkage of the lysyl oxidase-like (LOXL1) gene to the PML gene on human chromosome 15q22. Academic Article uri icon

Overview

abstract

  • A contig was constructed centered on the PML (promyelocytic leukemia) gene. Using an exon-trapping approach to identify potential genes from a pool of cosmids located 5' of the PML gene, four exons were identified that showed 100% sequence homology with the previously cloned lysyl oxidase-like (LOXL1) gene. An exon probe identified a single transcript of 2.4 kb on a multitissue Northern blot with a pattern identical to the one reported for the LOXL1 gene. Pulsed-field gel electrophoresis showed comigrating bands for both the PML cDNA and LOXL1 probes, demonstrating a physical linkage between these two genes. These data provide physical mapping information to complement the previous cytogenetic localization of LOXL1.

publication date

  • January 1, 2000

Research

keywords

  • Chromosomes, Human, Pair 15
  • Contig Mapping
  • Genetic Linkage
  • Neoplasm Proteins
  • Nuclear Proteins
  • Protein-Lysine 6-Oxidase
  • Transcription Factors

Identity

Scopus Document Identifier

  • 0034022532

Digital Object Identifier (DOI)

  • 10.1159/000015477

PubMed ID

  • 10773658

Additional Document Info

volume

  • 88

issue

  • 1-2