Detection of TCR-gamma gene rearrangements in early mycosis fungoides by non-radioactive PCR-SSCP. Academic Article uri icon

Overview

abstract

  • Early mycosis fungoides (MF) can mimic numerous benign inflammatory dermatoses on routine histological examination. In this study, a recently developed non-radioactive polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) technique was used to assess T-cell clonality in paraffin-embedded skin biopsies clinically and pathologically suspicious for early MF. Non-radioactive PCR-SSCP is a simple, sensitive, reproducible and rapid procedure requiring minimal instrumentation. DNA was extracted from 22 skin biopsies of 20 patients with suspected patch stage MF and 15 skin biopsies of inflammatory dermatoses. V gamma1-8, V gamma9, V gamma10, V gamma11 and J gamma1/J gamma2 consensus primers were used for T-cell receptor (TCR)-gamma gene rearrangement amplification. PCR products were analyzed by non-radioactive SSCP. Clonal TCR-gamma gene rearrangements were detected in 17 of 22 (77%) suspected MF specimens. Clonal SSCP banding patterns were different among individual patients. In addition, identical banded patterns were demonstrated in serial skin biopsies from the same patient. No dominant T-cell clones were found in the inflammatory dermatoses studied. Therefore, non-radioactive PCR-SSCP is a useful molecular diagnostic tool for assessment of T-cell clonality in paraffin-embedded specimens suspicious for early MF. The SSCP imprint of PCR products is specific for each TCR-gamma gene rearrangement, and may be used to evaluate concurrent/recurrent disease in individual patients.

publication date

  • May 1, 2000

Research

keywords

  • Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
  • Mycosis Fungoides
  • Receptors, Antigen, T-Cell, gamma-delta
  • Skin Neoplasms

Identity

Scopus Document Identifier

  • 0034098083

Digital Object Identifier (DOI)

  • 10.1034/j.1600-0560.2000.027005228.x

PubMed ID

  • 10847547

Additional Document Info

volume

  • 27

issue

  • 5