Retroviral-mediated gene transfer in primary murine and human T-lymphocytes. Academic Article uri icon

Overview

abstract

  • Recombinant retroviruses are efficient vectors for introducing genes into many mammalian cell types. They are useful in the context of clinical as well as experimental applications, owing to the ability to generate high-titer and helper-free viral stocks. Retroviral vectors are especially appropriate for the transduction of primary lymphocytes, because gene transfer is stable and mediated by nonimmunogenic vectors. Stable integration in chromosomes of cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells' progeny. However, oncoretroviral vectors derived from murine leukemia viruses (MLV) require target cell division to integrate. Here we review factors that determine retroviral-mediated gene transfer efficiency in primary T-lymphocytes, in particular, T-cell activation status, viral receptor expression, and culture conditions.

publication date

  • June 1, 2000

Research

keywords

  • Genetic Vectors
  • Retroviridae
  • T-Lymphocytes
  • Transfection

Identity

Scopus Document Identifier

  • 0033858961

Digital Object Identifier (DOI)

  • 10.1385/MB:15:2:133

PubMed ID

  • 10949826

Additional Document Info

volume

  • 15

issue

  • 2