Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils. Academic Article uri icon

Overview

abstract

  • Interleukin-9 (IL-9) has been implicated in the pathogenesis of allergic disorders. To examine the interaction between IL-9 and eosinophils, we evaluated mature peripheral blood eosinophils for their expression of the specific alpha-subunit of the IL-9 receptor (IL-9R-alpha). The expression of IL-9R-alpha by human eosinophils was detected at the messenger RNA (mRNA) and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, and immunocytochemical analysis, respectively. Functional analyses demonstrated that recombinant human (rh)IL-9 inhibited in vitro peripheral blood human eosinophil apoptosis in a concentration-dependent manner. We then examined the role of IL-9 in eosinophil differentiation using the human cord blood CD34(+) cells and human promyelocytic leukemia cells (HL-60). The addition of IL-9 to CD34(+) cells cultured in IL-3 and IL-5 enhanced eosinophil development, and IL-9 alone induced the expression of IL-5R-alpha. IL-9 also up-regulated the IL-5R-alpha chain cell surface expression during terminal eosinophil differentiation of the HL-60 cell line. Our findings suggest that IL-9 may potentiate in vivo eosinophil function by increasing their survival and IL-5-mediated differentiation and maturation. Taken together, these results suggest a mechanism by which IL-9 potentiates airway and tissue eosinophilia.

authors

  • Coleman, Morton
  • Gounni, A S
  • Gregory, B
  • Nutku, E
  • Aris, F
  • Latifa, K
  • Minshall, E
  • North, J
  • Tavernier, J
  • Levit, R
  • Nicolaides, N
  • Robinson, D
  • Hamid, Q

publication date

  • September 15, 2000

Research

keywords

  • Eosinophils
  • Interleukin-9
  • Receptors, Interleukin

Identity

Scopus Document Identifier

  • 0034665658

PubMed ID

  • 10979962

Additional Document Info

volume

  • 96

issue

  • 6