Detecting gene copy number fluctuations in tumor cells by microarray analysis of genomic representations. Academic Article uri icon

Overview

abstract

  • In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived from low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed.

publication date

  • November 1, 2000

Research

keywords

  • Breast Neoplasms
  • Gene Dosage
  • Oligonucleotide Array Sequence Analysis

Identity

PubMed Central ID

  • PMC310939

Scopus Document Identifier

  • 0033674221

Digital Object Identifier (DOI)

  • 10.1101/gr.138300

PubMed ID

  • 11076858

Additional Document Info

volume

  • 10

issue

  • 11