RNA helicase dynamics in pre-mRNA splicing. Academic Article uri icon

Overview

abstract

  • The DExH-box NTPase/helicase Prp22p plays two important roles in pre-mRNA splicing. It promotes the second transesterification reaction and then catalyzes the ATP-dependent release of mature mRNA from the spliceosome. Evidence that helicase activity is important emerged from the analysis of Prp22p motif III (SAT) mutations that uncouple the NTPase and helicase activities. We find that S635A and T637A hydrolyse ATP, but are defective in unwinding duplex RNA and releasing mRNA from the spliceosome. The S635A mutation is lethal in vivo at

publication date

  • December 1, 2000

Research

keywords

  • Fungal Proteins
  • RNA Helicases
  • RNA Splicing
  • RNA, Messenger
  • Saccharomyces cerevisiae Proteins

Identity

PubMed Central ID

  • PMC305851

Scopus Document Identifier

  • 0034423411

Digital Object Identifier (DOI)

  • 10.1093/emboj/19.23.6582

PubMed ID

  • 11101530

Additional Document Info

volume

  • 19

issue

  • 23