T cell receptor (TCR)-mediated repertoire selection and loss of TCR vbeta diversity during the initiation of a CD4(+) T cell response in vivo. Academic Article uri icon

Overview

abstract

  • We recently described a novel way to isolate populations of antigen-reactive CD4(+) T cells with a wide range of reactivity to a specific antigen, using immunization with a fixed dose of nominal antigen and FACS((R)) sorting by CD4(high) expression. Phenotypic, FACS((R)), functional, antibody inhibition, and major histocompatibility complex-peptide tetramer analyses, as well as T cell receptor Vbeta sequence analyses, of the antigen-specific CD4(high) T cell populations demonstrated that a diverse sperm whale myoglobin 110-121-reactive CD4(+) T cell repertoire was activated at the beginning (day 3 after immunization) of the immune response. Within 6 d of immunization, lower affinity clones were lost from the responding population, leaving an expanded population of oligoclonal, intermediate affinity (and residual high affinity) T cells. This T cell subset persisted for at least 4 wk after immunization and dominated the secondary immune response. These data provide evidence that CD4(+) T cell repertoire selection occurs early in the immune response in vivo and suggest that persistence and expansion of a population of oligoclonal, intermediate affinity T cells is involved in CD4(+) T cell memory.

publication date

  • December 18, 2000

Research

keywords

  • CD4-Positive T-Lymphocytes
  • Complementarity Determining Regions
  • Lymphocyte Activation
  • Receptors, Antigen, T-Cell

Identity

PubMed Central ID

  • PMC2213496

Scopus Document Identifier

  • 0034684648

Digital Object Identifier (DOI)

  • 10.1084/jem.192.12.1719

PubMed ID

  • 11120769

Additional Document Info

volume

  • 192

issue

  • 12