Ganglioside agglutination immunoassay for rapid detection of autoantibodies in immune-mediated neuropathy. Academic Article uri icon

Overview

abstract

  • Elevated levels of serum autoantibodies directed against gangliosides are closely associated with acute and chronic autoimmune neuropathies. An agglutination immunoassay using polystyrene microparticles coated with a total extract of brain gangliosides was used to test patient sera for the presence of anti-ganglioside antibodies. Results were compared with those obtained by ELISA for anti-GM1 and anti-GQ1b ganglioside antibodies. Eight of the twelve sera from patients with multifocal motor neuropathy and seven of the thirteen sera from patients with Guillain-BarrĂ© syndrome were positive for the presence of anti-ganglioside antibodies by the ganglioside agglutination immunoassay. The assay compared favorably with the ELISA system in sensitivity and specificity, while requiring a fraction of the time and cost to perform. The new assay can serve as a rapid and effective method for detecting or screening for anti-ganglioside antibodies in patients with acute or chronic immune-mediated neuropathies. It would be particularly useful for detecting antibodies that react with multiple gangliosides, or with minor or as yet uncharacterized gangliosides.

publication date

  • January 1, 2001

Research

keywords

  • Agglutination
  • Autoantibodies
  • Autoimmune Diseases of the Nervous System
  • G(M1) Ganglioside
  • Gangliosides

Identity

PubMed Central ID

  • PMC6808095

Scopus Document Identifier

  • 0035056614

Digital Object Identifier (DOI)

  • 10.1002/jcla.8

PubMed ID

  • 11291112

Additional Document Info

volume

  • 15

issue

  • 2