cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network. Academic Article uri icon

Overview

abstract

  • It is well established that Rho-GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF-dependent manner, similarly to coat proteins involved in vesicle formation and to several actin-binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans-Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase-deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.

publication date

  • May 1, 2001

Research

keywords

  • cdc42 GTP-Binding Protein
  • trans-Golgi Network

Identity

PubMed Central ID

  • PMC125445

Scopus Document Identifier

  • 0035341316

Digital Object Identifier (DOI)

  • 10.1093/emboj/20.9.2171

PubMed ID

  • 11331583

Additional Document Info

volume

  • 20

issue

  • 9