A sensitive fluorometric assay for tissue transglutaminase. Academic Article uri icon

Overview

abstract

  • We have devised a highly sensitive fluorometric well plate assay for tissue transglutaminase that is suitable for multiple kinetic analyses/high-throughput screening of chemical inventories for inhibitors of this enzyme. The procedure measures the rate of fluorescence enhancement (lambda(exc) 260 nm, lambda(em) 538 nm) when 1-N-(carbobenzoxy-l-glutaminylglycyl)-5-N-(5'N'N'-dimethylaminonaphthalenesulfonyl)diamidopentane (glutaminyl substrate) is cross-linked to dansyl cadaverine (amine substrate). The assay procedure can be used to measure the activity of as little as 60 microU of purified guinea pig liver tissue transglutaminase (4.2 ng or 54 fmol of enzyme).

publication date

  • May 15, 2001

Research

keywords

  • Enzyme Inhibitors
  • GTP-Binding Proteins
  • Liver
  • Transglutaminases

Identity

Scopus Document Identifier

  • 0035873616

Digital Object Identifier (DOI)

  • 10.1006/abio.2001.5040

PubMed ID

  • 11355851

Additional Document Info

volume

  • 292

issue

  • 2