Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages. Academic Article uri icon

Overview

abstract

  • Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.

publication date

  • May 11, 2001

Research

keywords

  • Isoenzymes
  • Macrophages
  • Prostaglandin-Endoperoxide Synthases
  • Transcription, Genetic
  • Up-Regulation

Identity

Scopus Document Identifier

  • 0035844046

Digital Object Identifier (DOI)

  • 10.1016/s0014-5793(01)02422-x

PubMed ID

  • 11356200

Additional Document Info

volume

  • 496

issue

  • 2-3