Biological methods for cell-cycle synchronization of mammalian cells. Review uri icon

Overview

abstract

  • Understanding the molecular and biochemical basis of cellular growth and division involves the investigation of regulatory events that most often occur in a cell-cycle phase-dependent fashion. Studies examining cell-cycle regulatory mechanisms and progression invariably require cell-cycle synchronization of cell populations. Thus, many methods have been established to synchronize cells at specific phases of the cell cycle. Several of the common methods involve pharmacological agents, which act at various points throughout the cell cycle. Because of adverse cellular perturbations resulting from many of the synchronizing drugs used, other synchrony methods that involve less perturbation of biological systems, such as serum deprivation, contact inhibition, and centrifugal elutriation have a significant advantage. The advantages and disadvantages of these cell synchronization methods are discussed in this review.

publication date

  • June 1, 2001

Research

keywords

  • Cell Cycle
  • Eukaryotic Cells
  • Mammals

Identity

Scopus Document Identifier

  • 0034983262

Digital Object Identifier (DOI)

  • 10.2144/01306rv01

PubMed ID

  • 11414226

Additional Document Info

volume

  • 30

issue

  • 6