A method for efficient isotopic labeling of recombinant proteins. Academic Article uri icon

Overview

abstract

  • A rapid and efficient approach for preparing isotopically labeled recombinant proteins is presented. The method is demonstrated for 13C labeling of the C-terminal domain of angiopoietin-2, 15N labeling of ubiquitin and for 2H/13C/15N labeling of the Escherichia coli outer-membrane lipoprotein Lpp-56. The production method generates cell mass using unlabeled rich media followed by exchange into a small volume of labeled media at high cell density. Following a short period for growth recovery and unlabeled metabolite clearance, the cells are induced. The expression yields obtained provide a fourfold to eightfold reduction in isotope costs using simple shake flask growths.

publication date

  • May 1, 2001

Research

keywords

  • Isotope Labeling
  • Lipoproteins
  • Recombinant Fusion Proteins

Identity

Scopus Document Identifier

  • 0034992645

Digital Object Identifier (DOI)

  • 10.1023/a:1011254402785

PubMed ID

  • 11430757

Additional Document Info

volume

  • 20

issue

  • 1