Stt4 PI 4-kinase localizes to the plasma membrane and functions in the Pkc1-mediated MAP kinase cascade. Academic Article uri icon

Overview

abstract

  • Production of the essential phospholipid PI4P at the Golgi by the Pik1 kinase is required for protein secretion, while a distinct pool of PI4P generated by the Stt4 kinase is critical for normal actin cytoskeleton organization. We identify a transmembrane protein that stabilizes Stt4 at the plasma membrane where it directs synthesis of PI4P, which is required for activation of the Rho1/Pkc1-mediated MAP kinase cascade. Inactivation of Stt4 or the PI4P 5-kinase Mss4 results in mislocalization of the Rho-GTPase GEF Rom2. Rom2 binds PI4,5P(2) through its PH domain and represents the first identified effector in the Stt4-Mss4 pathway. Based on these results, we propose that Stt4-Mss4 generates PI4,5P(2) at the plasma membrane, required to recruit/activate effector proteins such as Rom2.

publication date

  • May 1, 2002

Research

keywords

  • 1-Phosphatidylinositol 4-Kinase
  • Fungal Proteins
  • Mitogen-Activated Protein Kinases
  • Phosphotransferases

Identity

Scopus Document Identifier

  • 0036001334

Digital Object Identifier (DOI)

  • 10.1016/s1534-5807(02)00168-5

PubMed ID

  • 12015967

Additional Document Info

volume

  • 2

issue

  • 5