Measurements of ATP in mammalian cells. Academic Article uri icon

Overview

abstract

  • Levels of phosphorylated adenosine nucleotides, including the universal energy carrier adenosine 5(')-triphosphate (ATP) and its metabolites adenosine 5(')-diphosphate (ADP) and adenosine 5(')-monophosphate (AMP), define the energy state in living cells and are dependent mainly on mitochondrial function. In this article, we describe a method based on the luciferase-luciferin system used to measure mitochondrial ATP synthesis continuously in permeabilized mammalian cells and mitochondria isolated from animal tissues. We also describe a technique that uses the expression of recombinant targeted luciferase to report ATP content in different cell compartments. Finally, we describe an HPLC-based method for accurate measurement of ATP, ADP, and AMP in cultured cells and animal tissues.

publication date

  • April 1, 2002

Research

keywords

  • Adenosine Triphosphate
  • Biochemistry
  • Mitochondria

Identity

Scopus Document Identifier

  • 0036024974

Digital Object Identifier (DOI)

  • 10.1016/S1046-2023(02)00037-3

PubMed ID

  • 12054922

Additional Document Info

volume

  • 26

issue

  • 4