Multilaboratory comparison of anaerobe susceptibility results using 3 different agar media. Academic Article uri icon

Overview

abstract

  • A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of >0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.

publication date

  • September 1, 2002

Research

keywords

  • Bacteria, Anaerobic
  • Culture Media
  • Microbial Sensitivity Tests

Identity

Scopus Document Identifier

  • 0036061232

Digital Object Identifier (DOI)

  • 10.1086/341919

PubMed ID

  • 12173107

Additional Document Info

volume

  • 35

issue

  • Suppl 1