Integrin-dependence of lymphocyte entry into the splenic white pulp. Academic Article uri icon

Overview

abstract

  • The steps involved in lymphocyte homing to the white pulp cords of the spleen are poorly understood. We demonstrate here that the integrins lymphocyte function associated (LFA)-1 and alpha 4 beta 1 make essential and mostly overlapping contributions necessary for B cell migration into white pulp cords. T cell entry to the white pulp is also reduced by blockade of LFA-1 and alpha 4 beta 1. The LFA-1 ligand, intercellular adhesion molecule 1 is critical for lymphocyte entry and both hematopoietic cells and radiation-resistant cells contribute to this requirement. Vascular cell adhesion molecule 1 contributes to the alpha 4 beta 1 ligand requirement and a second ligand, possibly fibronectin, also plays a role. By contrast with the entry requirements, antigen-induced movement of B cells from follicles to the outer T zone is not prevented by integrin blocking antibodies. Comparison of the distribution of integrin-blocked B cells and B cells treated with the G alpha i inhibitor, pertussis toxin, early after transfer reveals in both cases reduced accumulation in the inner marginal zone. These observations suggest that chemokine receptor signaling and the integrins LFA-1 and alpha 4 beta 1 function together to promote lymphocyte transit from the marginal zone into white pulp cords.

publication date

  • February 3, 2003

Research

keywords

  • Integrins
  • Lymphocytes
  • Spleen

Identity

PubMed Central ID

  • PMC2193837

Scopus Document Identifier

  • 0037415553

Digital Object Identifier (DOI)

  • 10.1084/jem.20021569

PubMed ID

  • 12566419

Additional Document Info

volume

  • 197

issue

  • 3