Whole CagA gene amplification of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism. Academic Article uri icon

Overview

abstract

  • To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.

publication date

  • January 1, 2002

Research

keywords

  • Antigens, Bacterial
  • Bacterial Proteins
  • Gene Amplification
  • Helicobacter pylori
  • Polymorphism, Restriction Fragment Length

Identity

Scopus Document Identifier

  • 3042833001

Digital Object Identifier (DOI)

  • 10.1007/BF02896762

PubMed ID

  • 12674756

Additional Document Info

volume

  • 22

issue

  • 4