Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication. Academic Article uri icon

Overview

abstract

  • We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)'s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

publication date

  • May 1, 2003

Research

keywords

  • Gene Expression Regulation, Viral
  • Hepacivirus
  • RNA, Viral
  • Viral Nonstructural Proteins
  • Virus Replication

Identity

PubMed Central ID

  • PMC154017

Scopus Document Identifier

  • 0038415985

Digital Object Identifier (DOI)

  • 10.1128/jvi.77.10.6055-6061.2003

PubMed ID

  • 12719597

Additional Document Info

volume

  • 77

issue

  • 10