Conditional immortalization of bicarbonate-secreting intercalated cells from rabbit. Academic Article uri icon

Overview

abstract

  • We have derived an immortalized cell line from primary cultures of bicarbonate-secreting intercalated cells from rabbit. Cells were transfected with a plasmid encoding a temperature-sensitive large T antigen of SV40 plus the neomycin resistance gene under the control of an SV40 promoter. Transfectants were selected for resistance to G418. One stably transfected clone, designated IC250, was subcloned to ensure clonality, and a subclone (clone C) was characterized in detail. The cells divide continuously at permissive temperature. At restrictive temperature, they cease dividing and assume morphological and transport properties of true bicarbonate-secreting intercalated cells. They express appropriate ultrastructural features, bind peanut lectin in an apical pattern, are rich in carbonic anhydrase, stain for proton-adenosinetriphosphatase in a basolateral pattern, and do not stain with antibodies to erythrocyte band 3. Most monolayers of transformed type B intercalated cells do not achieve a significant transepithelial resistance; those monolayers that are sufficiently electrically tight for electrophysiological studies are capable of chloride-dependent bicarbonate transport.

publication date

  • August 1, 1992

Research

keywords

  • Bicarbonates
  • Cell Line, Transformed
  • Kidney

Identity

Scopus Document Identifier

  • 0026660872

Digital Object Identifier (DOI)

  • 10.1152/ajpcell.1992.263.2.C521

PubMed ID

  • 1325122

Additional Document Info

volume

  • 263

issue

  • 2 Pt 1