Sequence-specific detection of MicroRNAs by signal-amplifying ribozymes. Academic Article uri icon

Overview

abstract

  • The rational and straightforward design of hairpin ribozymes that can be sequence-specifically induced by external oligonucleotides is described. Due to intrinsic signal amplification, their sensitivity is at least an order of magnitude increased compared to standard molecular beacons. We applied this system to the detection of microRNAs, a recently discovered class of small endogenous RNA molecules that are involved in gene regulation. We show that the cognate microRNA can reliably and sensitively be detected at low concentrations in a mix of other microRNA sequences. These probes may be useful in applications that require direct detection of minute amounts of small DNAs or RNAs.

publication date

  • January 28, 2004

Research

keywords

  • MicroRNAs
  • RNA, Catalytic

Identity

Scopus Document Identifier

  • 1642535356

Digital Object Identifier (DOI)

  • 10.1021/ja038822u

PubMed ID

  • 14733539

Additional Document Info

volume

  • 126

issue

  • 3