Discrimination of Listeria monocytogenes from other Listeria species by ligase chain reaction. Academic Article uri icon

Overview

abstract

  • A ligase chain reaction assay based on a single-base-pair difference in the V9 region of the 16S rRNA gene (16S rDNA) was developed to distinguish between Listeria monocytogenes and other Listeria species. For this purpose, two pairs of primers were designed, with one primer of each pair being radioactively labeled. The ligated product was separated from the primers by denaturing polyacrylamide gel electrophoresis and then detected by autoradiography. To achieve a higher sensitivity, the 16S rDNA was initially amplified by polymerase chain reaction prior to the ligase chain reaction. The ligase chain reaction was tested on 19 different Listeria species and strains and proved to be a highly specific diagnostic method for the detection of L. monocytogenes.

publication date

  • November 1, 1992

Research

keywords

  • DNA, Ribosomal
  • Listeria
  • Listeria monocytogenes
  • Nucleic Acid Amplification Techniques
  • RNA, Ribosomal, 16S

Identity

PubMed Central ID

  • PMC183127

Scopus Document Identifier

  • 0026496081

Digital Object Identifier (DOI)

  • 10.1128/aem.58.11.3443-3447.1992

PubMed ID

  • 1482171

Additional Document Info

volume

  • 58

issue

  • 11