NMR probing of in silico identification of anti-HPV16 E7 mAb linear peptide epitope. Academic Article uri icon

Overview

abstract

  • A proteomics-based approach was exploited in order to individuate peptide sequences having the immunogenic potential to evoke humoral response. The epitope search utilized two parameters: the similarity level of the peptide sequence to the host's proteins, and the peptide capability to bind to the major histocompatibility complex class II molecules. By this approach, the human papillomavirus 16 E7(49-63) RAHYNIVTFCCKCDS peptide was individuated as the immunogenic epitope recognized by an anti-HPV16 E7 monoclonal antibody raised against the full-length viral oncoprotein. In this report, two-dimensional nuclear magnetic resonance spectroscopic experiments unequivocally probe the HPV16 E7 epitope individuation.

publication date

  • February 1, 2004

Research

keywords

  • Computer Simulation
  • Epitopes
  • Molecular Mimicry
  • Oncogene Proteins, Viral
  • Papillomaviridae

Identity

Scopus Document Identifier

  • 1942451978

Digital Object Identifier (DOI)

  • 10.1016/j.peptides.2003.12.004

PubMed ID

  • 15063005

Additional Document Info

volume

  • 25

issue

  • 2