Measurement of cytokines in autoimmune disease. Academic Article uri icon

Overview

abstract

  • Systemic autoimmune diseases are characterized by extensive alterations in immune system function, with cytokines and autoantibodies contributing to impaired immunoregulation and tissue damage. Characterization of the expression and function of cytokines is important for elucidation of pathogenic mechanisms and for identification of therapeutic targets and strategies. We reviewed the utility of assays that reflect individual variability in cytokine gene sequence, expression of messenger ribonucleic acid (mRNA) or protein, as well as measurement of expression of target genes regulated by cytokines. Real-time reverse transcriptase polymerase chain reaction and intracellular staining for cytokine expression are two sensitive and quantitative approaches for analysis of cytokine mRNA and protein, respectively. Detailed methods are provided for these assays.

publication date

  • January 1, 2004

Research

keywords

  • Autoimmune Diseases
  • Cytokines

Identity

Scopus Document Identifier

  • 10444252299

Digital Object Identifier (DOI)

  • 10.1385/1-59259-805-6:129

PubMed ID

  • 15286384

Additional Document Info

volume

  • 102