Analysis of androgen regulated homeobox gene NKX3.1 during prostate carcinogenesis. Academic Article uri icon

Overview

abstract

  • PURPOSE: NKX3.1 is an androgen regulated gene that is largely specific to the prostate for expression and it is predicted to encode a homeobox protein. Null alleles of NKX3.1 in mice results in impaired prostate development as well as hyperplasia and dysplasia of the prostate. In addition, the NKX3.1 gene maps to a region of high loss of heterozygosity in prostate cancer in humans, suggesting that NKX3.1 might have a direct role in prostate carcinogenesis, possibly functioning as a tumor suppressor protein. Previous studies of the levels of NKX3.1 mRNA or protein in prostate cancer specimens have resulted in conflicting findings. MATERIALS AND METHODS: To resolve this issue we assessed NKX3.1 expression by mRNA in situ analysis and immunohistochemistry on the same prostate cancer tissue arrays. RESULTS: Data showed that NKX3.1 mRNA and protein levels in prostate cancer specimens are correlated, suggesting that most regulation is at the transcriptional level. There was no correlation of NKX3.1 expression levels with tumor grade or clinical stage. In general there was a suggestion that worse clinical features at surgery were associated with lower IHC stain scores. In particular, extracapsular extension but not seminal vesicle invasion inversely correlated with NKX3.1 expression. CONCLUSIONS: Together these data suggest that NKX3.1 does not function as a typical tumor suppressor protein in prostate cancer but it may still have important regulatory roles during prostate cancer progression.

publication date

  • September 1, 2004

Research

keywords

  • Androgens
  • Genes, Homeobox
  • Genes, Tumor Suppressor
  • Prostatic Neoplasms

Identity

Scopus Document Identifier

  • 4143112443

Digital Object Identifier (DOI)

  • 10.1097/01.ju.0000136526.78535.b8

PubMed ID

  • 15311057

Additional Document Info

volume

  • 172

issue

  • 3