Association between gene expression profile and tumor invasion in oral squamous cell carcinoma. Academic Article uri icon

Overview

abstract

  • There are limited studies attempting to correlate the expression changes in oral squamous cell carcinoma with clinically relevant variables. We determined the gene expression profile of 16 tumor and 4 normal tissues from 16 patients by means of Affymetrix Hu133A GeneChips. The hybridized RNA was isolated from cells obtained with laser capture microdissection, then was amplified and labeled using T7 polymerase-based in vitro transcription. The expression of 53 genes was found to differ significantly (33 upregulated, 20 downregulated) in normal versus tumor tissues under two independent statistical methods. The expression changes in four selected genes (LGALS1, MMP1, LAGY, and KRT4) were confirmed with reverse transcriptase polymerase chain reaction. Two-dimensional hierarchical clustering of the 53 genes resulted in the samples clustering according to the extent of tumor infiltration: normal epithelial tissue, tumors less than or equal to 4 cm in dimension, and tumors more than 4 cm in dimension (P = 0.0014). The same pattern of clustering was also observed for the 20 downregulated genes. We did not observe any associations with lymph node metastasis (P = 0.097).

publication date

  • October 1, 2004

Research

keywords

  • Carcinoma, Squamous Cell
  • Gene Expression Regulation, Neoplastic
  • Mouth Neoplasms

Identity

Scopus Document Identifier

  • 4644247108

Digital Object Identifier (DOI)

  • 10.1016/j.cancergencyto.2004.01.026

PubMed ID

  • 15381369

Additional Document Info

volume

  • 154

issue

  • 1