Study of T-cell costimulatory blockade in vivo at a single-cell level. Academic Article uri icon

Overview

abstract

  • The critical role of costimulatory signals in T-cell activation and the complexity of T-cell costimulatory pathways involved make a detailed understanding of this system a challenging task. By taking advantage of the unique chemical properties of CFSE, we and others have developed an in vivo model that allows quantitative analysis of T-cell activation at a single-cell level. This model involves labeling of donor T-cells with the tracking dye CFSE and adoptively transferring into lethally irradiated allogeneic hosts. T-cells proliferating in the host mice can be explicitly analyzed upon recovery. By using mice deficient for certain costimulatory molecules as a source of donor cells or by treating the host mice with reagents that block certain costimulatory pathways, this CFSE model is extremely useful in studying the role of T-cell costimulatory signals in activation, survival, and effector differentiation of alloreactive T-cells in vivo.

publication date

  • January 1, 2005

Research

keywords

  • Lymphocyte Activation
  • T-Lymphocytes

Identity

Scopus Document Identifier

  • 16644365955

Digital Object Identifier (DOI)

  • 10.1385/1-59259-862-5:409

PubMed ID

  • 15585935

Additional Document Info

volume

  • 109