Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication. Academic Article uri icon

Overview

abstract

  • For many viruses, primary infection has been shown to prevent superinfection by a homologous second virus. In this study, we investigated superinfection exclusion of bovine viral diarrhea virus (BVDV), a positive-sense RNA pestivirus. Cells acutely infected with BVDV were protected from superinfection by homologous BVDV but not with heterologous vesicular stomatitis virus. Superinfection exclusion was established within 30 to 60 min but was lost upon passaging of persistently infected cells. Superinfecting BVDV failed to deliver a translatable genome into acutely infected cells, indicating a block in viral entry. Deletion of structural protein E2 from primary infecting BVDV abolished this exclusion. Bypassing the entry block by RNA transfection revealed a second block at the level of replication but not translation. This exclusion did not require structural protein expression and was inversely correlated with the level of primary BVDV RNA replication. These findings suggest dual mechanisms of pestivirus superinfection exclusion, one at the level of viral entry that requires viral glycoprotein E2 and a second at the level of viral RNA replication.

publication date

  • March 1, 2005

Research

keywords

  • Diarrhea Viruses, Bovine Viral
  • RNA, Viral
  • Viral Interference
  • Virus Replication

Identity

PubMed Central ID

  • PMC1075699

Scopus Document Identifier

  • 14744286414

Digital Object Identifier (DOI)

  • 10.1128/JVI.79.6.3231-3242.2005

PubMed ID

  • 15731218

Additional Document Info

volume

  • 79

issue

  • 6