Concerted auto-regulation in yeast endosomal t-SNAREs. Academic Article uri icon

Overview

abstract

  • In yeast, the assembly of the target (t)-SNAREs [Tlg2p/Tlg1p,Vti1p] and [Pep12p/Tlg1p,Vti1p] with the vesicular (v)-SNARE Snc2p promotes endocytic fusion. Here, selected mutations and truncations of SNARE proteins were tested in an in vitro fusion assay to identify potential regulatory regions in these proteins, and two distinct regions were found. The first is represented by the combined effect of the three t-SNARE N-terminal regions and the second is located within the Tlg1p SNARE motif. These internal controls provide a potential mechanism to enable SNARE-dependent fusion to be regulated.

publication date

  • March 30, 2005

Research

keywords

  • Endosomes
  • Gene Expression Regulation, Fungal
  • Vesicular Transport Proteins

Identity

Scopus Document Identifier

  • 20444390976

Digital Object Identifier (DOI)

  • 10.1074/jbc.M500841200

PubMed ID

  • 15799968

Additional Document Info

volume

  • 280

issue

  • 22