Identification of early tolerance regulator genes induced by allochimeric therapy using microarray-based genomewide scan. Academic Article uri icon

Overview

abstract

  • We have demonstrated that peri- or postoperative delivery of allochimeric [a1h(u)]-RT1.A(a) class I major histocompatibility complex molecules with donor-type (RT1A(u)) immunogenic epitopes presented in recipient-type (RT1A(a)) sequences induced donor-specific tolerance in ACI (RT1a) recipients of WF (RT1u) heart allografts. A genomic scan during the early posttransplant period was performed to elucidate the underlying operative mechanisms. A rat genome study after transplantation was carefully designed using Affymetrix Rat Genome 230 2.0 Array. The allochimeric treatment group is 3-day cyclosporine (CsA)-treated ACI recipients that accepted Wistar Furth RT1u cardiac allografts with postoperative dosage of allochimeric molecules, while the control is 3-day CsA-treated ACI recipients of WF cardiac allografts. All the samples were harvested 5 days after heart transplant as the early stage of tolerance detection. Following array data normalization and modeling, we compared the above two treatment groups and identified a total of 250 tolerance regulator genes induced by allochimeric molecules only.

publication date

  • May 1, 2005

Research

keywords

  • Gene Expression Regulation
  • Genome
  • Heart Transplantation
  • Immune Tolerance
  • Oligonucleotide Array Sequence Analysis
  • Transplantation Chimera

Identity

Scopus Document Identifier

  • 20344385997

Digital Object Identifier (DOI)

  • 10.1016/j.transproceed.2005.03.147

PubMed ID

  • 15919512

Additional Document Info

volume

  • 37

issue

  • 4