An automated procedure to properly handle digital images in large scale tissue microarray experiments. Academic Article uri icon

Overview

abstract

  • Tissue Microarray (TMA) methodology has been recently developed to enable "genome-scale" molecular pathology studies. To enable high-throughput screening of TMAs automation is mandatory, both to speed up the process and to improve data quality. In particular, in acquiring digital images of single tissues (core sections) a crucial step is the correct recognition of each tissue position in the array. In fact, further reliable data analysis is based on the exact assignment of each tissue to the corresponding tumor. As most of the times tissue alignment in the microarray grid is far from being perfect, simple strategies to perform proper acquisition do not fit well. The present paper describes a new solution to automatically perform grid location assignment. We developed an ad hoc image processing procedure and a robust algorithm for object recognition. Algorithm accuracy tests and assessment of working constraints are discussed. Our approach speeds up TMA data collection and enables large scale investigation.

publication date

  • September 1, 2005

Research

keywords

  • Automation
  • Image Processing, Computer-Assisted
  • Tissue Array Analysis

Identity

Scopus Document Identifier

  • 23944487757

Digital Object Identifier (DOI)

  • 10.1016/j.cmpb.2005.04.004

PubMed ID

  • 15979757

Additional Document Info

volume

  • 79

issue

  • 3