Inositol 1,4,5-trisphosphate receptor phosphorylation in breast cancer. Academic Article uri icon

Overview

abstract

  • The aim of this study was to establish the type(s) of inositol 1,4,5-trisphosphate receptors (IP3Rs) in T47D breast cancer cells that regulate intracellular calcium (Ca2+) and whether they interact with cyclin (Cy), an important regulator of cyclin-dependent kinases (cdk), during cell cycle progression. Immunoblotting, immunoprecipitation, and pull-down assays were used to identify IP3R expression and interaction with Cy. The relative IP3R3 expression, as compared to IP3R1, was higher in these cells. Pull-down analysis showed that IP3R3 interacted with both CyA and CyB. The interaction with Cys and the phosphorylation of IP3Rs by Cy/cdk complexes provide a novel mechanism of regulating intracellular Ca2+ release and Ca2+-dependent signaling events in breast cancer.

publication date

  • July 6, 2005

Research

keywords

  • Breast Neoplasms
  • Calcium Channels
  • Cyclins
  • Protein Isoforms
  • Receptors, Cytoplasmic and Nuclear

Identity

Scopus Document Identifier

  • 24644478128

Digital Object Identifier (DOI)

  • 10.1159/000086954

PubMed ID

  • 16006774

Additional Document Info

volume

  • 26

issue

  • 4