Tomographic fluorescence mapping of tumor targets. Academic Article uri icon

Overview

abstract

  • Methods that allow robust imaging of specific molecular targets and biological processes in vivo should have widespread applications in biology and clinical medicine. Here we use a quantitative, three-dimensional fluorescence-mediated tomographic technique (FMT) that enables rapid measurements of fluorochrome-based affinity tags in live xenograft models. We validate the method by showing its sensitivity in quantitating tumor angiogenesis and therapeutic modulation using an anti-vascular endothelial growth factor antibody. Furthermore, we show the feasibility of simultaneous multichannel measurements of distinct biological phenomena such as receptor tyrosine kinase expression and angiogenesis. FMT measurements can be done serially, with short imaging times and within the same live animal. The described method should be valuable for rapidly profiling biological phenomena in vivo.

publication date

  • July 15, 2005

Research

keywords

  • Breast Neoplasms
  • Gliosarcoma
  • Tomography, Optical

Identity

Scopus Document Identifier

  • 22244458589

Digital Object Identifier (DOI)

  • 10.1158/0008-5472.CAN-05-0382

PubMed ID

  • 16024635

Additional Document Info

volume

  • 65

issue

  • 14