p130Cas mediates the transforming properties of the anaplastic lymphoma kinase. Academic Article uri icon

Overview

abstract

  • Translocations of the anaplastic lymphoma kinase (ALK) gene have been described in anaplastic large-cell lymphomas (ALCLs) and in stromal tumors. The most frequent translocation, t(2;5), generates the fusion protein nucleophosmin (NPM)-ALK with intrinsic tyrosine kinase activity. Along with transformation, NPM-ALK induces morphologic changes in fibroblasts and lymphoid cells, suggesting a direct role of ALK in cell shaping. In this study, we used a mass-spectrometry-based proteomic approach to search for proteins involved in cytoskeleton remodeling and identified p130Cas (p130 Crk-associated substrate) as a novel interactor of NPM-ALK. In 293 cells and in fibroblasts as well as in human ALK-positive lymphoma cell lines, NPM-ALK was able to bind p130Cas and to induce its phosphorylation. Both of the effects were dependent on ALK kinase activity and on the adaptor protein growth factor receptor-bound protein 2 (Grb2), since no binding or phosphorylation was found with the kinase-dead mutant NPM-ALK(K210R) or in the presence of a Grb2 dominant-negative protein. Phosphorylation of p130Cas by NPM-ALK was partially independent from Src (tyrosine kinase pp60c-src) kinase activity, as it was still detectable in Syf-/- cells. Finally, p130Cas-/- (also known as Bcar1-/-) fibroblasts expressing NPM-ALK showed impaired actin filament depolymerization and were no longer transformed compared with wild-type cells, indicating an essential role of p130Cas activation in ALK-mediated transformation.

publication date

  • August 16, 2005

Research

keywords

  • Cell Transformation, Neoplastic
  • Crk-Associated Substrate Protein
  • Cytoskeleton
  • Oncogene Proteins, Fusion
  • Protein-Tyrosine Kinases

Identity

PubMed Central ID

  • PMC1895100

Scopus Document Identifier

  • 28444462500

Digital Object Identifier (DOI)

  • 10.1182/blood-2005-03-1204

PubMed ID

  • 16105984

Additional Document Info

volume

  • 106

issue

  • 12