Effects of hepatocyte growth factor and platelet-derived growth factor on the repair of meniscal defects in vitro. Academic Article uri icon

Overview

abstract

  • Injuries to the avascular region of the meniscus occur frequently and may be difficult to repair. This study was designed to determine whether growth factors could diffuse from a collagen sponge or a collagen gel into meniscal tissue and stimulate healing of defects using an in vitro model. The diffusion of platelet-derived growth factor (PDGF) from the collagen carriers into the medium was rapid with approximately 50% being released from the collagen sponge within the first hour. After 5 d of incubation, 8% of the PDGF was present in the meniscus, 11% in the collagen sponge, and 62% had been released into the medium. Similar results were obtained when a collagen gel was used as a carrier. Histological evaluation of the meniscal explants after 2 wk in culture revealed extensive proteoglycan staining in the areas surrounding defects treated with either hepatocyte growth factor (HGF) or PDGF compared with controls without growth factor. The HGF-PDGF treatment resulted in alignment and migration of meniscal cells toward the defect, which was not observed in untreated controls. At 3-7 d, increased number of cells were observed in defects treated with collagen gels (but not the sponge) with PDGF-HGF. At 4 wk, combined HGF-PDGF treatment resulted in the formation of tissue with birefringence by polarized microscopy, suggestive of organized collagen. The data suggest that use of specific PDGF-HGF may enhance the repair of meniscal injuries.

publication date

  • January 1, 2005

Research

keywords

  • Drug Delivery Systems
  • Hepatocyte Growth Factor
  • Platelet-Derived Growth Factor
  • Tibial Meniscus Injuries
  • Wound Healing

Identity

Scopus Document Identifier

  • 30844452026

Digital Object Identifier (DOI)

  • 10.1290/0503018.1

PubMed ID

  • 16409118

Additional Document Info

volume

  • 41

issue

  • 8-9