Virally-directed fluorescent imaging (VFI) can facilitate endoscopic staging. Academic Article uri icon

Overview

abstract

  • BACKGROUND: Replication-competent, tumor specific herpes simplex virus NV1066 expresses green fluorescent protein (GFP) in infected cancer cells. We sought to determine the feasibility of GFP-guided imaging technology in the intraoperative detection of small tumor nodules. METHODS: Human cancer cell lines were infected with NV1066 at multiplicities of infection of 0.01, 0.1 and 1. Cancer cell specific infectivity, vector spread and GFP signal intensity were measured by flow cytometry and time-lapse digital imaging (in vitro); and by use of a stereomicroscope and endoscope equipped with a fluorescent filter (in vivo). RESULTS: NV1066 infected all cancer cell lines and expressed GFP at all MOIs. GFP signal was significantly higher than the autofluorescence of normal cells. One single dose of NV1066 spread within and across body cavities and selectively infected tumor nodules sparing normal tissue. Tumor nodules undetectable by conventional thoracoscopy and laparoscopy were identified by GFP fluorescence. CONCLUSION: Virally-directed fluorescent imaging (VFI) is a real-time novel molecular imaging technology that has the potential to enhance the intraoperative detection of endoluminal or endocavitary tumor nodules.

publication date

  • January 30, 2006

Research

keywords

  • Green Fluorescent Proteins
  • Herpes Simplex
  • Luminescent Agents
  • Neoplasms
  • Oncolytic Viruses
  • Simplexvirus

Identity

PubMed Central ID

  • PMC1435379

Scopus Document Identifier

  • 33645729035

Digital Object Identifier (DOI)

  • 10.1007/s00464-005-0259-6

PubMed ID

  • 16446989

Additional Document Info

volume

  • 20

issue

  • 4