Adiponectin stimulates proliferation and cytokine secretion in colonic epithelial cells. Academic Article uri icon

Overview

abstract

  • Adiponectin is a recently described mediator secreted by adipose tissue. Here we report the growth promoting and pro-inflammatory actions of adiponectin on colonic epithelial cancer cells. Full-length and globular adiponectin produced an identical stimulation of HT-29 cell growth that was blocked by inhibition of adenylate cyclase and protein kinase A and partially inhibited by a pan-specific protein kinase C inhibitor, but was unaffected by specific inhibition of extracellular signal-related kinase (ERK) or p38 MAP kinase. Globular adiponectin but not full-length adiponectin significantly increased the secretion and mRNA levels of IL-8, GM-CSF and MCP-1. Globular adiponectin doubled IL-1beta-stimulated IL-8 and GM-CSF secretion. Adiponectin-stimulated cytokine secretion was blocked by pharmacological inhibitors of NF-kappaB, ERK and p38 MAP kinase. Globular adiponectin increased phosphorylation of both ERK and p38 MAP kinase and increased the nuclear translocation of active NF-kappaB. Adiponectin has pro-proliferative and pro-inflammatory actions on colonic epithelial cells; these appear to be differentially activated by the adiponectin isoforms. Adiponectin may have a role in the regulation of gastrointestinal mucosal function, inflammation and colon carcinogenesis.

publication date

  • March 10, 2006

Research

keywords

  • Adiponectin
  • Cell Proliferation
  • Cytokines
  • Intestinal Mucosa

Identity

Scopus Document Identifier

  • 33646190053

Digital Object Identifier (DOI)

  • 10.1016/j.regpep.2006.02.001

PubMed ID

  • 16529829

Additional Document Info

volume

  • 134

issue

  • 2-3