The role of Staphylococcus aureus adhesins in the pathogenesis of ventricular assist device-related infections. Academic Article uri icon

Overview

abstract

  • Ventricular assist devices (VADs) are an important form of therapy for end-stage congestive heart failure. However, infection of the VAD, which is often caused by Staphylococcus aureus, poses a major threat to survival. Using a novel in vitro binding assay with VAD membranes and a heterologous lactococcal system of expression, we identify 3 S. aureus proteins--clumping factor A (ClfA) and fibronectin binding proteins A and B (FnBPA and FnBPB) as the main factors involved in adherence to VAD polyurethane membranes. Adherence is greatly diminished by long implantation times, reflecting a change in topological features of the VAD membrane, and is primarily mediated by the FnBPA domains in the staphylococcal proteins. We also compare the adherence of S. aureus mutant strains and show that other staphylococcal components appear to be involved in adherence to VAD membranes. Finally, we demonstrate that ClfA, FnBPA, and FnBPB mediate bacterial infection of implanted murine intra-aortic polyurethane patches.

publication date

  • March 2, 2006

Research

keywords

  • Adhesins, Bacterial
  • Coagulase
  • Heart-Assist Devices
  • Prosthesis-Related Infections
  • Staphylococcal Infections
  • Staphylococcus aureus

Identity

Scopus Document Identifier

  • 33645760425

Digital Object Identifier (DOI)

  • 10.1086/501366

PubMed ID

  • 16544251

Additional Document Info

volume

  • 193

issue

  • 8