Sildenafil induces angiogenic response in human coronary arteriolar endothelial cells through the expression of thioredoxin, hemeoxygenase and vascular endothelial growth factor. Academic Article uri icon

Overview

abstract

  • This study was undertaken to investigate the effect of phosphodiesterase-5 (PDE5) inhibitor, sildenafil, on angiogenic response in human coronary arteriolar endothelial cells (HCAEC). The cells exposed to sildenafil (1-20 microM) demonstrated significantly accelerated tubular morphogenesis with the induction of thioredoxin-1 (Trx-1), hemeoxygenase-1 (HO-1) and VEGF. Sildenafil induced VEGF and angiopoietin specific receptors such as KDR, Tie-1 and Tie-2. This angiogenic response was repressed by tinprotoporphyrin IX (SnPP), an inhibitor of HO-1 enzyme activity. Sildenafil below 1 muM has no angiogenic effect as evidenced by reduced tuborogenesis. Sildenafil along with SnPP inhibited both VEGF and Angiopoietin-1 (Ang-1) protein expression. Therefore our results demonstrated for the first time that sildenafil is a very potent pro-angiogenic factor.

publication date

  • May 22, 2006

Research

keywords

  • Endothelial Cells
  • Heme Oxygenase (Decyclizing)
  • Neovascularization, Physiologic
  • Piperazines
  • Thioredoxins
  • Vascular Endothelial Growth Factor A

Identity

Scopus Document Identifier

  • 33748521847

Digital Object Identifier (DOI)

  • 10.1016/j.vph.2006.03.010

PubMed ID

  • 16716755

Additional Document Info

volume

  • 45

issue

  • 2