Construction and characterization of soluble, cleaved, and stabilized trimeric Env proteins based on HIV type 1 Env subtype A. Academic Article uri icon

Overview

abstract

  • The generation of an antibody response capable of neutralizing a broad range of clinical isolates remains an important goal of human immunodeficiency virus type 1 (HIV-1) vaccine development. Envelope glycoprotein (Env)-based vaccine candidates will also need to take into account the extensive genetic diversity of circulating HIV-1 strains. We describe here the generation of soluble, stabilized, proteolytically cleaved, trimeric forms of Env (SOSIP gp140 proteins) based on contemporary Env subtype A viruses from East Africa. We discuss issues associated with the construction, purification, and characterization of such complex proteins; not all env sequences allow the expression of trimeric proteins. However, stabilized trimers from one such protein, KNH1144 SOSIP gp140, were successfully made. These proteins are now being prepared for preclinical immunogenicity studies.

publication date

  • June 1, 2006

Research

keywords

  • Gene Products, env
  • HIV Antibodies

Identity

Scopus Document Identifier

  • 33745726588

Digital Object Identifier (DOI)

  • 10.1089/aid.2006.22.569

PubMed ID

  • 16796532

Additional Document Info

volume

  • 22

issue

  • 6