Enhanced sensitivity and stability in two-color in situ hybridization by means of a novel chromagenic substrate combination. Academic Article uri icon

Overview

abstract

  • Double in situ hybridization analysis is a fundamental technique for studying the expression of two genes with high temporal and spatial resolution. However, due to the lack of sensitivity in current detection methods, this approach is powerful only when at least one transcript is abundantly expressed. Here, we report a new enzyme/chromagenic substrate combination that provides sufficient sensitivity for detecting two less abundant transcripts and stability for subsequent paraffin sectioning.

publication date

  • October 1, 2006

Research

keywords

  • Chromogenic Compounds
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization

Identity

Scopus Document Identifier

  • 33749255401

Digital Object Identifier (DOI)

  • 10.1002/dvdy.20909

PubMed ID

  • 16894600

Additional Document Info

volume

  • 235

issue

  • 10