Septic arthritis. Staphylococcal induction of chondrocyte proteolytic activity. Academic Article uri icon

Overview

abstract

  • We report herein that cartilage proteolytic activity increased in bovine and rabbit articular cartilage after treatment with a purified staphylococcal culture medium or intraarticular infection with Staphylococcus aureus. Staphylococcal culture medium increased the release of gelatinolytic, collagenolytic, and caseinolytic activity into the medium of isolated chondrocytes or cartilage organ culture. The proteolytic activities were determined in assays using radiolabeled substrate and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Staphylococcal culture medium was proteolytically inactive by both assay techniques. RNA synthesis of isolated chondrocytes was unaffected by staphylococcal culture medium, whereas overall protein synthesis was inhibited by 84%. An analysis of extracts of Staphylococcus aureus-infected rabbit knee cartilage by substrate gels showed increased gelatinolytic and caseinolytic activity compared with extracts of uninfected knee cartilage. Our data suggest that the rapid loss of proteoglycan and persistent degradation of cartilage in staphylococcal septic arthritis is due to the production and activation of chondrocyte proteases.

publication date

  • April 1, 1990

Research

keywords

  • Arthritis, Infectious
  • Cartilage, Articular
  • Endopeptidases
  • Metalloendopeptidases
  • Staphylococcal Infections
  • Staphylococcus aureus

Identity

Scopus Document Identifier

  • 0025194077

Digital Object Identifier (DOI)

  • 10.1002/art.1780330411

PubMed ID

  • 1691643

Additional Document Info

volume

  • 33

issue

  • 4