An enzymatic assay for vitronectin based on its selective phosphorylation by protein kinase A. Academic Article uri icon

Overview

abstract

  • The catalytic subunit (C) of cAMP-dependent protein kinase selectively phosphorylates vitronectin, a plasma protein that promotes cell adhesion and platelet aggregation, inhibits the inactivation of thrombin by antithrombin III, and participates in complement function. This specific phosphorylation is used here (a) to develop an enzymatic assay for vitronectin (with C and [gamma-32P]ATP) which can be used to identify the vitronectin-containing fractions at each stage of its purification; (b) to radioactively label vitronectin and differentiate between the intact and the nicked form of this protein in structure-function studies; and (c) to identify possible vitronectin-related proteins in the plasma of other animal species.

publication date

  • August 1, 1990

Research

keywords

  • Blood Proteins
  • Glycoproteins
  • Protein Kinases

Identity

Scopus Document Identifier

  • 0025161409

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(90)90608-c

PubMed ID

  • 1699453

Additional Document Info

volume

  • 188

issue

  • 2