Phosphoinositide synthesis and degradation in isolated rat liver peroxisomes. Academic Article uri icon

Overview

abstract

  • Analyzing peroxisomal phosphoinositide (PId(#)) synthesis in highly purified rat liver peroxisomes we found synthesis of phosphatidylinositol 4-phosphate (PtdIns4P), PtdIns(4,5)P(2) and PtdIns(3,5)P(2). PtdIns3P was hardly detected in vitro, however, was observed in vivo after [(32)P]-phosphate labeling of primary rat hepatocytes. In comparison with other subcellular organelles peroxisomes revealed a unique PId pattern suggesting peroxisomal specificity of the observed synthesis. Use of phosphatase inhibitors enhanced the amount of PtdIns4P. The results obtained provide evidence that isolated rat liver peroxisomes synthesize PIds and suggest the association of PId 4-kinase and PId 5-kinase and PId 4-phosphatase activities with the peroxisomal membrane.

publication date

  • October 5, 2006

Research

keywords

  • Hepatocytes
  • Peroxisomes
  • Phosphatidylinositols

Identity

Scopus Document Identifier

  • 33750072352

Digital Object Identifier (DOI)

  • 10.1016/j.febslet.2006.09.058

PubMed ID

  • 17045591

Additional Document Info

volume

  • 580

issue

  • 25